Top 10 genomenewsnetwork.org Competitors

dnaftb.org

discover the concepts and experiments that define the fields of genetics and molecular biology. this animated primer features the work of over 100 scientists and researchers.

genomebiology.com

Background The oocyte-to-embryo transition (OET) converts terminally differentiated gametes into a totipotent embryo and is critically controlled by maternal mRNAs and proteins, while the genome is silent until zygotic genome activation. How the transcriptome, translatome, and proteome are coordinated during this critical developmental window remains poorly understood. Results Utilizing a highly sensitive and quantitative mass spectrometry approach, we obtain high-quality proteome data spanning seven mouse stages, from full-grown oocyte (FGO) to blastocyst, using 100 oocytes/embryos at each stage. Integrative analyses reveal distinct proteome reprogramming compared to that of the transcriptome or translatome. FGO to 8-cell proteomes are dominated by FGO-stockpiled proteins, while the transcriptome and translatome are more dynamic. FGO-originated proteins frequently persist to blastocyst while corresponding transcripts are already downregulated or decayed. Improved concordance between protein and translation or transcription is observed for genes starting translation upon meiotic resumption, as well as those transcribed and translated only in embryos. Concordance between protein and transcription/translation is also observed for proteins with short half-lives. We built a kinetic model that predicts protein dynamics by incorporating both initial protein abundance in FGOs and translation kinetics across developmental stages. Conclusions Through integrative analyses of datasets generated by ultrasensitive methods, our study reveals that the proteome shows distinct dynamics compared to the translatome and transcriptome during mouse OET. We propose that the remarkably stable oocyte-originated proteome may help save resources to accommodate the demanding needs of growing embryos. This study will advance our understanding of mammalian OET and the fundamental principles governing gene expression.

dnalc.org

dna, genetics, and biotechnology hands-on educational programs and online resources for students, educators, and the public.

yourgenome.org

This animation shows the steps in the polymerase chain reaction that scientists use to make lots of copies of a DNA sample.

genome.gov

The Genomics and Health Disparities Interest Group provides a forum to connect individuals from different scientific disciplines across the National Institutes of Health (NIH) and researchers in the Washington D.C. metropolitan area engaged in genomics and health disparities research, as well as provide opportunities for professional development, networking and community engagement.

jcvi.org

Join our team JCVI is a premier, not-for-profit genomics research institute with more than 120 scientists and staff in...

accessexcellence.org

a place in cyberspace for biology, health and life science teachers and learners.

completegenomics.com

At the American Society for Human Genetics in Washington D.C., geneticists worldwide were briefed on Complete Genomics’ DNBSEQ revolutionizing full genome sequencing, the use of DNBSEQ-T7 in clinical labs for structural variant detection, and a University of Massachusetts researcher’s use of STOmics’s Stereo-Seq and DNBSEQ-T7 data. DNBSEQ technology and new advances in whole genome sequencing...