Buffers vs. HPLC Advisor Utilizzo e statistiche
Buffers is a tool for designing buffer solutions for pH control. Buffers is useful both as a handy reference of available buffering agents and as an accurate, portable buffer calculator for chemical, biochemical and biological research.
With Buffers you can:
- Select from 42 commonly used buffering agents
- Generate accurate recipes covering broad pH, concentration and temperature ranges
- Access detailed information about each available buffer system
- E-mail, text, copy or print recipes
To use Buffers, just choose a buffering agent and target pH along with the desired solution volume, temperature and buffer concentration. The recipe is calculated with the push of a button. Buffers uses a sophisticated algorithm that is more versatile and potentially more accurate than simple calculations based on the Henderson-Hasselbalch equation.
An information page for each buffering agent lists the pKa, appropriate pH range, and temperature dependence, along with molecular weights and CAS numbers for the acid and base forms and other pertinent information.
Bring your buffer calculations into the lab where you need them!
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The Agilent InfinityLab HPLC Advisor app offers tools to save you time with HPLC troubleshooting, method development, and more. Once installed, it works also offline—no matter if you are next to or away from the instrument. Moreover, these tools work for all HPLC instruments, regardless of brand and model.
Troubleshooting
Typical HPLC problems are summarized and organized in groups—so you can quickly define the issue in a couple clicks.
For each problem, you can choose to view all potential issues for tips or get guided step-by-step help to troubleshoot your issue. This flexible app offers two ways to help users solve their HPLC problems.
Calculators
Method translation
This calculator helps you translate your legacy methods to new columns and systems. Simply enter the information from your legacy method (column, system, experimental conditions, and gradient) along with the new column and system that you are going to use. Then, the calculator determines the experimental conditions and the gradient of your new translated method. For all fields in these calculators, you can either use default values or values that are specific to your method, column, and system. All results can be saved as a PDF.
Chromatographic performance
This calculator helps you predict how a chromatographic method will perform. Fill in parameters such as column geometry, system dwell volume, mobile phase, experimental conditions, etc. Then, this app will calculate the expected chromatographic performance (e.g., gradient slope, number of plates, peak capacity, backpressure, optimal flow rate). For all fields in these calculators, you can either use default values or values that are specific to your method, column, and system. All results can be saved as a PDF.
Data Library
Conversions
This section shows you LC-related information, such as the conversion factors between different units, details of selected physical constants, powers of ten, and concentration values.
Formulas
This section lists LC-related formulas. The search function helps you find formulas easily and quickly. All formulas, as well as all related parameters, are listed and linked to other related formulas if applicable.
Learn More
This section contains select Agilent webpages for you to get more HPLC-related information.
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Buffers VS. 
HPLC Advisorgennaio 5, 2025